Macroscopic and histopathological study of the placenta: an essential resource in litigation processes

The pathological study of the placenta is of upmost importance in cases of unexplained fetal/perinatal loss and often these carry litigation implications. Integrating pathological findings and the underlying pathophysiological processes, leading to placental lesions, is fundamental for the evaluation of poor fetal and perinatal outcomes and to distinguish from cases of true negligence

Understanding oral mucositis: a pilot study

info:eu-repo/semantics/acceptedVersio

Development, characterisation, and consumer acceptance of an innovative vegan burger with seaweed

This work was funded by national funds through FCT—Fundação para a Ciência e a Tecnologia, I.P., within the scope of the project MARE (UIDB/04292/2020 and UIDP/04292/2020) and the project LA/P/0069/2020 granted to the Associate Laboratory ARNET. This study was financially supported by ProReMar project (MAR-04,03,01-FEAMP-0380) funded by the European Maritime and Fisheries Fund under the Operational Program Mar 2020/Nacional.What consumers choose when purchasing food is of most importance to promote sustainability. The consumption of more sustainable foods should be stimulated, for example, by using more sustainable ingredients and by consumer education. Therefore, an innovative and highly nutritious vegan burger with seaweed (VBS) was developed using sustainable ingredients, such as pulses—grass pea (Lathyrus sativus L.) and chickpea (Cicer arietinum L.)—and the seaweed Dulse (Palmaria palmata L.) from aquaculture. VBS was analysed for its physico-chemical and nutritional characteristics, including antioxidant activity (DPPH, TPC) and fatty acid and mineral element profiles. Shelf life and consumer acceptability were determined. The VBS was shown to be a source of protein (8.01 ± 0.14% fresh weight (FW)), fibre (5.75% FW), and mineral elements, such as P, Fe, rich in Mg, Mn, and Cu, while having low sodium content. Moreover, it presents a low sugar content. Furthermore, no antioxidant activity was detected. The pasteurised and vacuum-packed product had a shelf life of 90 days and was well accepted by consumers (64.0% acceptance). It may be concluded that an innovative VBS, nutritionally rich and with a shelf life of 90 days, was developed and well accepted by consumers, which is a good addition to a rich and diverse diet.info:eu-repo/semantics/publishedVersio

Quality assessment of chilled and frozen fish: mini review

Fish is a very perishable food and therefore several storage strategies need to be employed to increase its shelf-life, guaranteeing its safety and quality from catch to consumption. Despite the advances in modern fish storage technologies, chilling and freezing are still the most common preservation methods used onboard. The present review aims to summarize strategies to increase the shelf-life of fresh (chilled) and frozen fish, as whole, gutted, or fillet, involving the assessment of different traditional cooling and freezing conditions of di erent fish species caught in different locations. Although there are other factors that influence the fish shelf-life, such as the fish species and the stress suffered during catch, storage time and temperature and the amount of ice are some of the most important. In addition, the way that fish is stored (whole, fillet, or gutted) also contributes to the final quality of the product. In most studies, whole chilled and frozen fish present longer shelf-life than those preserved as gutted and filleted. However, it should be noted that other factors related to the organism, capture method, and transport to the preparation/processing industry should be considered for shelf-life extension.info:eu-repo/semantics/publishedVersio

Release of volatile compounds from polymeric microcapsules mediated by photocatalytic nanoparticles

In this study we propose a suitable method for the solar-activated controlled release of volatile compounds from polymeric microcapsules bonded with photocatalytic nanoparticles. These reservoirs can find applications, for example, in the controlled release of insecticides, repellents, or fragrances, amongst other substances. The surfaces of the microcapsules have been functionalized with TiO2 nanoparticles.Upon ultraviolet irradiation, redox mechanisms are initiated on the semiconductor surface resulting in the dissociation of the polymer chains of the capsule wall and, finally, volatilization of the encapsulated compounds. The quantification of the output release has been performed by gas chromatography analysis coupled with mass spectroscopy.Strategic Project PEST-C/FIS/UI607/2011 and PTDC/CTMNAN/119979/2010 Projec

Mineral content and volatile profiling of Prunus avium L. (sweet cherry) y-Products from Fundão Region (Portugal)

Large amounts of Prunus avium L. by-products result from sweet cherry production and processing. This work aimed to evaluate the mineral content and volatile profiling of the cherry stems, leaves, and flowers of the Saco cultivar collected from the Fundão region (Portugal). A total of 18 min erals were determined by ICP-MS, namely 8 essential and 10 non-essential elements. Phosphorus (P) was the most abundant mineral, while lithium (Li) was detected in trace amounts. Three different preparations were used in this work to determine volatiles: hydroethanolic extracts, crude extracts, and aqueous infusions. A total of 117 volatile compounds were identified using HS-SPME/GC MS, distributed among different chemical classes: 31 aldehydes, 14 alcohols, 16 ketones, 30 esters, 4 acids, 4 monoterpenes, 3 norisoprenoids, 4 hydrocarbons, 7 heterocyclics, 1 lactone, 1 phenol, and 2 phenylpropenes. Benzaldehyde, 4-methyl-benzaldehyde, hexanal, lilac aldehyde, and 6-methyl 5-hepten-2-one were the major volatile compounds. Differences in the types of volatiles and their respective amounts in the different extracts were found. This is the first study that describes the mineral and volatile composition of Portuguese sweet cherry by-products, demonstrating that they could have great potential as nutraceutical ingredients and natural flavoring agents to be used in the pharmaceutical, cosmetic, and food industries.info:eu-repo/semantics/publishedVersio

Continuous production of highly tuned silk/calcium-based composites: exploring new pathways for skin regeneration

Calcium plays an important role in barrier function repair and skin homeostasis. In particular, calcium phosphates (CaPs) are well established materials for biomedical engineering due to their biocompatibility. To generate biomaterials with a more complete set of biological properties, previously discarded silk sericin (SS) has been recovered and used as a template to grow CaPs. Crucial characteristics for skin applications, such as antibacterial activity, can be further enhanced by doping CaPs with cerium (Ce) ions. The effectiveness of cell attachment and growth on the materials highly depends on their morphology, particle size distribution, and chemical composition. These characteristics can be tailored through the application of oscillatory flow technology, which provides precise mixing control of the reaction medium. Thus, in the present work, CaP/SS and CaP/SS/Ce particles were fabricated for the first time using a modular oscillatory flow plate reactor (MOFPR) in a continuous mode. Furthermore, the biological behavior of both these composites and of previously produced pure CaPs was assessed using human dermal fibroblasts (HDFs). It was demonstrated that both CaP based with plate-shaped nanoparticles and CaP-SS-based composites significantly improved cell viability and proliferation over time. The results obtained represent a first step towards the reinvention of CaPs for skin engineering.info:eu-repo/semantics/publishedVersio

Synergistic activity of a novel class of azoimidazole dyes with polyvinylpyrrolidone-silver nanoparticles for the development of antibacterial textiles

The high demand for novel antimicrobial textiles by the medical, health care, hygiene, sportswear, personal protective equipment, and filtration sectors promoted the growth of functional textiles. However, the efficacy of antimicrobial agents against different pathogens is a considerable challenge due to the distinctive mechanisms of action and resistance. The development of novel synergistic antimicrobial agents may offer numerous opportunities to enhance antimicrobial effectiveness, namely boost the activity of individual agents, reduce dosages, minimize toxicity, and amplify the activity spectrum. On the one hand, azo dyes containing a heterocycle present good tinctorial strength and brightness of shades. In particular, the imidazole ring also has interesting antimicrobial, analgesic, and anti-inflammatory properties. On the other hand, silver nanoparticles (AgNPs) are renowned antimicrobial agents against a wide range of microorganisms, but their application is limited by the toxicity observed for effective concentrations. In this work, a novel class of azoimidazoles (AzoIz) and corresponding precursors (AmIz) were conjugated with polyvinylpyrrolidone-coated AgNPs, and their synergistic effect was assessed against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The results showed interesting antimicrobial properties of the novel AzoIz molecules when combined with a very small concentration of AgNPs. Thus, the application of these conjugates in textiles may lead to highly colored materials with remarkable antimicrobial properties, which worth to be further explored

Synthesis and antimicrobial activity of a new class of azoimidazoles

The emergence of infectious diseases caused by new pathogens or multidrug-resistant (MDR) strains has been a global health threat over the last decades.1 These infections are among the most severe healthcare problems and have been associated to several deaths and heavy economic burden per year.2,3 The imidazole ring is present in several natural and synthetic molecules with biological activity namely on effective antimicrobial agents, which make it a vital anchor for the development of new therapeutic molecules in this field.4 Furthermore, amidrazones are known for their high reactivity thus being useful intermediates for the synthesis of compounds with a wide range of biological activities including antimicrobial. The amidrazone derivatives have been applied in different subjects of chemistry, specifically in the synthesis of azo molecules.5 In a previous work, novel imidazole-based 5-aminoimidazole-4-carboxamidrazones were prepared and exhibited potent antimicrobial activity against C. krusei and C. albicans.6 Further biological studies to elucidate the action mechanism revealed an interesting relationship between the antimicrobial activity and total intracellular ROS production by the yeasts.7 As these carboxamidrazones had previously evidenced a particular susceptibility to the presence of oxygen, all of these results combined prompted us to study the reactivity of 5-aminoimidazole-4-carboxamidrazones in the presence of different oxidant and antioxidant agents. Here, we present results of the electrochemical characterization by cyclic voltammetry to elucidate the oxidation mechanism of these compounds, and the results of the attempts performed to oxidize amidrazones in order to obtain the corresponding azoimidazoles. These products were fully characterized by NMR spectroscopy (including 1H RMN, D2O shake, 13C RMN, HMQC, HMBC and NOE techniques), mass spectroscopy, ATR-FTIR, melting point and elemental analysis. The antimicrobial activity of these new products has been also evaluated and highly promising results were obtained. All the results will be presented and discussed.Fundação para a Ciência e a Tecnologia (FCT) for financial support through the Chemistry Research Centre of the University of Minho (UID/QUI/00686/2020) and CIIMAR (UIBD/04423/2020). This work was also supported under the projects MEDCOR (PTDC/CTM-TEX/1213/2020) and UID/CTM/00264/2019, and the PhD grant SFRH/BD/137668/201

Seasonal sensory evaluation of low commercial value or unexploited fish species from the Portuguese coast

Overfishing is increasing over time, and according to FAO (Food and Agriculture Organization), about one third of the world’s fish stocks are now overfished. Thus, diversifying the target species is essential for fisheries sustainability contributing to improve resource-effcient processes. Non-target species can be valuable resources for the development of new food products. However, those species are scarcely studied, and it is of high importance to trace their seasonal sensory profile as a first step towards their valorisation. Therefore, in this study, seasonal influence on sensory properties of five low commercial value or unexploited fish species, namely Trachurus picturatus (blue jack mackerel), Spondyliosoma cantharus (black seabream), Trigla lyra (piper gurnard), Serranus cabrilla (comber) and Capros aper (boarfish), was assessed in order to identify the most favourable season for catching each species. Fish samples were assessed by a panel of 16 semi-trained assessors for sensory attributes previously identified. The evaluation takes place every 2 months. Statistical differences were reported between attributes and seasons for all species, except for T. lyra, which did not present any di erence in its sensory attributes throughout the year.info:eu-repo/semantics/publishedVersio